研究業績

2023年

原著論文

• "Alternative splicing of a single exon causes a major impact on the affinity of Caenorhabditis elegans tropomyosin isoforms for actin filaments."
  Shoichiro Ono, Eichi Watabe, Keita Morisaki, Kanako Ono, Hidehito Kuroyanagi.
  Frontiers in Cell and Developmental Biology 11, 1208913 (2023).

  • DOI: 10.3389/fcell.2023.1208913
• "Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification."
  Hirotatsu Imai, Daisuke Utsumi, Hidetsugu Torihara, Kenzo Takahashi, Hidehito Kuroyanagi, Akio Yamashita.
  Nucleic Acids Research 51, 14, e76 (2023).

  • DOI: 10.1093/nar/gkad545
• "Structure of the Caenorhabditis elegans m6A methyltransferase METT10 that regulates SAM homeostasis."
  Jue Ju, Tomohiko Aoyama, Yuka Yashiro, Seisuke Yamashita, Hidehito Kuroyanagi, Kozo Tomita.
  Nucleic Acids Research 51, 5, 2434–2446 (2023).

  • DOI: 10.1093/nar/gkad081

学会発表

• "ヒトの拡張型心筋症変異を模したrbm20変異ゼブラフィッシュの作製"
  島村杏、鳥原英嗣
  グローバルサイエンスキャンパス(GSC) 令和５年度 全国受講生研究発表会 (2023).
• "ゼブラフィッシュを用いたrbm20によるタイチンのスプライシング制御の進化的保存性"
  島村杏
  日本進化学会第25回大会 (2023).
• "ゼブラフィッシュを用いたrbm20によるタイチンのスプライシング制御の進化的保存性"
  島村杏
  沖縄科学技術教育シンポジウム2023 (2023).

2022年

原著論文

• "I536T variant of RBM20 affects splicing of cardiac structural proteins that are causative for developing dilated cardiomyopathy."
  Takuma Yamamoto, Rie Sano, Aya Miura, Mai Imasaka, Yoshiro Naito, Minori Nishiguchi, Kensuke Ihara, Naruhito Otani, Yoshihiko Kominato, Masaki Ohmuraya, Hidehito Kuroyanagi and Hajime Nishio.
  Journal of Molecular Medicine 100, 12, 1741–1754 (2022).

  • DOI: 10.1007/s00109-022-02262-8

総説

• "ナンセンスコドン介在的mRNA分解（NMD）と共役した選択的スプライシングによる遺伝子発現の制御"
  黒柳秀人
  生化学 94, 6, 868-874 (2022).

  • DOI: 10.14952/SEIKAGAKU.2022.940868

書籍分担執筆

• "第3章. 分子と遺伝学「スプライシング」"
  黒柳秀人
  遺伝学の百科事典 継承と多様性の源（日本遺伝学会編）（丸善出版） ISBN: 978-4-621-30660-4 (2022).

  • URL

2021年

原著論文

• "Periostin Exon-21 Antibody Neutralization of Triple-Negative Breast Cancer Cell-Derived Periostin Regulates Tumor-Associated Macrophage Polarization and Angiogenesis."
  Tatsuya Fujikawa, Fumihiro Sanada, Yoshiaki Taniyama, Kana Shibata, Naruto Katsuragi, Nobutaka Koibuchi, Kaori Akazawa, Yuko Kanemoto, Hidehito Kuroyanagi, Kenzo Shimazu, Hiromi Rakugi and Ryuichi Morishita.
  Cancers 13, 20, 5072 (2021).

  • DOI: 10.3390/cancers13205072
• "Loss of Caenorhabditis elegans homologue of human MOB4 compromises life span, health life span and thermotolerance."
  Momotaj Jahan, Hiroaki Iwasa, Hidehito Kuroyanagi, Yutaka Hata.
  Genes to Cells 26, 10, 798-806 (2021).

  • URL
  • DOI: 10.1111/gtc.12891
• "m6A-mediated alternative splicing coupled with nonsense-mediated mRNA decay regulates SAM synthetase homeostasis."
  Eichi Watabe, Marina Togo-Ohno, Yuma Ishigami, Shotaro Wani, Keiko Hirota, Mariko Kimura-Asami, Sharmin Hasan, Satomi Takei, Akiyoshi Fukamizu, Yutaka Suzuki, Tsutomu Suzuki, Hidehito Kuroyanagi.
  The EMBO Journal 40, e106434 (2021).

  • URL
  • DOI: 10.15252/embj.2020106434

2020年

原著論文

• "Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs."
  Jay W Schneider, Saji Oommen, Muhammad Y Qureshi, Sean C Goetsch, David R Pease, Rhianna S Sundsbak, Wei Guo, Mingming Sun, Han Sun, Hidehito Kuroyanagi, Dennis A Webster, Alexander W Coutts, Kimberly A Holst, Brooks S Edwards, Nikolas Newville, Matthew A Hathcock, Tamene Melkamu, Francesca Briganti, Wu Wei, Maria G Romanelli, Scott C Fahrenkrug, Doug E Frantz, Timothy M Olson, Lars M Steinmetz, Daniel F Carlson, Timothy J Nelson, Wanek Program Preclinical Pipeline.
  Nature Medicine 26, 11, 1788–1800 (2020).

  • DOI: 10.1038/s41591-020-1087-x
• "A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice."
  Kensuke Ihara, Tetsuo Sasano, Yuichi Hiraoka, Marina Togo-Ohno, Yurie Soejima, Motoji Sawabe, Megumi Tsuchiya, Hidesato Ogawa, Tetsushi Furukawa, Hidehito Kuroyanagi.
  Scientific Reports 10, 17894 (2020).

  • DOI: 10.1038/s41598-020-74800-8

総説

• "mRNA Editing, Processing and Quality Control in Caenorhabditis elegans."
  Joshua A Arribere, Hidehito Kuroyanagi, Heather A Hundley.
  Genetics 215, 3, 531-568 (2020).

  • DOI: 10.1534/genetics.119.301807

2018年

原著論文

• "Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner"
  Eichi Watabe, Shoichiro Ono, Hidehito Kuroyanagi
  Cytoskeleton (Hoboken) 75, 10, 427-436 (2018).

  • DOI: 10.1002/cm.21489
• "UNC119 is a binding partner of tumor suppressor Ras-association domain family 6 and induces apoptosis and cell cycle arrest by MDM2 and p53"
  Hiroaki Iwasa, Aradhan Sarkar, Takanobu Shimizu, Takeru Sawada, Shakhawoat Hossain, Xiaoyin Xu, Junichi Maruyama, Kyoko Arimoto-Matsuzaki, Kanchanamala Withanage, Kentaro Nakagawa, Hidetake Kurihara, Hidehito Kuroyanagi, Yutaka Hata
  Cancer Science 109, 9, 2767-2780 (2018).

  • DOI: 10.1111/cas.13706
• "Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization."
  Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura & Hidehito Kuroyanagi.
  Scientific Reports 8, 8970 (2018).

  • DOI: 10.1038/s41598-018-26624-w
• "Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans"
  Dawn E Barnes, Eichi Watabe, Kanako Ono, Euiyoung Kwak, Hidehito Kuroyanagi, Shoichiro Ono
  Molecular Biology of the Cell 29, 9, 1075-1088 (2018).

  • DOI: 10.1091/mbc.E17-03-0152

総説

• "Alternative Splicing Regulator RBM20 and Cardiomyopathy."
  Takeshi Watanabe, Akinori Kimura, Hidehito Kuroyanagi.
  Frontiers in Molecular Biosciences 5, 105 (2018).

  • DOI: 10.3389/fmolb.2018.00105

2017年

総説

• "An emerging model organism Caenorhabditis elegans for alternative pre-mRNA processing in vivo"
  Shotaro Wani, Hidehito Kuroyanagi
  Wiley Interdisciplinary Reviews RNA 8, 6, e1428 (2017).

  • DOI: 10.1002/wrna.1428

書籍分担執筆

• "第9章第4節: 転写と転写後プロセシングの共役"
  黒柳秀人
  遺伝子発現制御機構 ―クロマチン，転写制御，エピジェネティクス―（田村隆明・浦聖恵 編）（東京化学同人）, 910-914 (2017).

  • URL

2016年

原著論文

• "Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor"
  Masahiro Tomioka, Yasuki Naito, Hidehito Kuroyanagi, Yuichi Iino
  Nature Communications 7, 11645 (2016).

  • DOI: 10.1038/ncomms11645
• "Evolutionarily conserved autoregulation of alternative pre-mRNA splicing by ribosomal protein L10a"
  Satomi Takei, Marina Togo-Ohno, Yutaka Suzuki, Hidehito Kuroyanagi
  Nucleic Acids Research 44, 12, 5585-5596 (2016).

  • DOI: 10.1093/nar/gkw152

書籍分担執筆

• "第26章: デコイ非コードRNA"
  黒柳 秀人
  ノンコーディングRNA―RNA分子の全体像を俯瞰する―（廣瀬哲郎・泊幸秀 編）（化学同人）, 278-289 (2016).

  • URL

2014年

原著論文

• "RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing"
  Kanako Kuwasako, Mari Takahashi, Satoru Unzai, Kengo Tsuda, Seiko Yoshikawa, Fahu He, Naohiro Kobayashi, Peter Güntert, Mikako Shirouzu, Takuhiro Ito, Akiko Tanaka, Shigeyuki Yokoyama, Masatoshi Hagiwara, Hidehito Kuroyanagi, Yutaka Muto
  Nature Structural & Molecular Biology 21, 778–786 (2014).

  • DOI: 10.1038/nsmb.2870
• "Comprehensive analysis of mutually exclusive alternative splicing in C. elegans"
  Hidehito Kuroyanagi, Satomi Takei & Yutaka Suzuki
  Worm 3, 1, e28459 (2014).

  • DOI: 10.4161/worm.28459

総説

• "組織特異的にスプライシング反応を制御するRBFOXファミリーRNA結合タンパク質およびSUP-12によるRNAの協働的な認識の機構"
  桑迫香奈子・高橋真梨・黒柳秀人・武藤 裕
  ライフサイエンス 新着論文レビュー (2014).

  • DOI: 10.7875/first.author.2014.117

2013年

原著論文

• "CELF family RNA-binding protein UNC-75 regulates two sets of mutually exclusive exons of the unc-32 gene in neuron-specific manners in Caenorhabditis elegans."
  Hidehito Kuroyanagi, Yohei Watanabe, Masatoshi Hagiwara.
  PLoS Genetics 9, 2, e1003337 (2013).

  • DOI: 10.1371/journal.pgen.1003337
• "Position-dependent and neuron-specific splicing regulation by the CELF family RNA-binding protein UNC-75 in Caenorhabditis elegans"
  Hidehito Kuroyanagi, Yohei Watanabe, Yutaka Suzuki, Masatoshi Hagiwara
  Nucleic Acids Research 41, 7, 4015–4025 (2013).

  • DOI: 10.1093/nar/gkt097
• "Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans"
  Hiroaki Iwasa, Sainawaer Maimaiti, Hidehito Kuroyanagi, Shodai Kawano, Kazutoshi Inami, Shikshya Timalsina, Mitsunobu Ikeda, Kentaro Nakagawa, Yutaka Hata
  Experimental Cell Research 319, 7, 931-945 (2013).

  • DOI: 10.1016/j.yexcr.2013.01.020
• "Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1"
  Hiroaki Iwasa, Hidehito Kuroyanagi, Sainawaer Maimaiti, Mitsunobu Ikeda, Kentaro Nakagawa, Yutaka Hata
  Experimental Cell Research 319, 3, 1-11 (2013).

  • DOI: 10.1016/j.yexcr.2012.10.008

総説

• "Switch-like regulation of tissue-specific alternative pre-mRNA processing patterns revealed by customized fluorescence reporters"
  Hidehito Kuroyanagi
  Worm 2, 3, e23834 (2013).

  • DOI: 10.4161/worm.23834
• "New Technology 選択的スプライシング可視化システム"
  木村まり子・黒柳秀人
  Medical Science Digest 39, 11, 502-503 (2013).

  • URL

2012年

原著論文

• "Muscle-specific splicing factors ASD-2 and SUP-12 cooperatively switch alternative pre-mRNA processing patterns of the ADF/cofilin gene in Caenorhabditis elegans"
  Genta Ohno, Kanako Ono, Marina Togo, Yohei Watanabe, Shoichiro Ono, Masatoshi Hagiwara, Hidehito Kuroyanagi
  PLoS Genetics 8, 10, e1002991 (2012).

  • DOI: 10.1371/journal.pgen.1002991

書籍分担執筆

• "Chapter 28 Single-Cell Detection of Splicing Events with Fluorescent Splicing Reporters"
  Hidehito Kuroyanagi, Akihide Takeuchi, Takayuki Nojima, Masatoshi Hagiwara
  Alternative pre‐mRNA Splicing: Theory and Protocols (Prof. Dr. Stefan Stamm, Prof. Dr. Christopher W. J. Smith, Prof. Dr. Reinhard Lührmann Eds.) (Wiley‐VCH Verlag GmbH & Co. KGaA), 298-309 (2012).

  • DOI: 10.1002/9783527636778.ch28

2010年

原著論文

• "Dual role of the carboxyl-terminal region of pig liver L-kynurenine 3-monooxygenase: mitochondrial-targeting signal and enzymatic activity"
  Kumiko Hirai, Hidehito Kuroyanagi, Yoshitaka Tatebayashi, Yoshitaka Hayashi, Kanako Hirabayashi-Takahashi, Kuniaki Saito, Seiich Haga, Tomihiko Uemura, Susumu Izumi
  The Journal of Biochemistry 148, 6, 639-650 (2010).

  • DOI: 10.1093/jb/mvq099
• "Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans"
  Hidehito Kuroyanagi, Genta Ohno, Hiroaki Sakane, Hiroyuki Maruoka, Masatoshi Hagiwara
  Nature Protocols 5, 9, 1495–1517 (2010).

  • DOI: 10.1038/nprot.2010.107

総説

• "みにれびゅー「選択的pre-mRNAスプライシング制御機構解明への新たなアプローチ」"
  黒柳秀人
  生化学 82, 5, 402-411 (2010).

書籍分担執筆

• "第3章第1節：選択的スプライシングの分子機構と生命現象"
  大野源太／黒柳秀人
  実験医学増刊 拡大・進展を続けるRNA研究の最先端（塩見春彦，塩見美喜子，稲田利文，廣瀬哲郎／編）（羊土社） 28, 10 , 1591-1596 (2010).

  • URL

2009年

総説

• "Fox-1 family of RNA-binding proteins"
  Hidehito Kuroyanagi
  Cellular and Molecular Life Sciences 66, 24, 3895-3907 (2009).

  • DOI: 10.1007/s00018-009-0120-5

書籍分担執筆

• "選択的スプライシングの可視化と制御機構解明への応用"
  黒柳秀人
  蛋白質核酸酵素増刊 mRNAプログラム―多様性と非対称性の獲得戦略―（稲田利文・大野睦人 編集）（共立出版） 54, 16 Suppl, 2044-2048 (2009).

  • URL

2008年

原著論文

• "STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo"
  Genta Ohno, Masatoshi Hagiwara, Hidehito Kuroyanagi
  Genes & Development 22, 3, 360-374 (2008).

  • DOI: 10.1101/gad.1620608

2007年

原著論文

• "The Fox-1 family and SUP-12 coordinately regulate tissue-specific alternative splicing in vivo"
  Hidehito Kuroyanagi, Genta Ohno, Shohei Mitani, Masatoshi Hagiwara
  Molecular and Cellular Biology 27, 24, 8612-8621 (2007).

  • DOI: 10.1128/MCB.01508-07
• "The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans"
  Keiko Gengyo-Ando, Hidehito Kuroyanagi, Tetsuo Kobayashi, Motohide Murate, Kazushi Fujimoto, Shigeo Okabe, Shohei Mitani
  EMBO Reports 8, 2, 152-157 (2007).

  • DOI: 10.1038/sj.embor.7400882

総説

• "HOTPRESS「選択的スプライシングを生体内で可視化する」"
  黒柳秀人・萩原正敏
  細胞工学 26, 1, 64-65 (2007).

  • URL

2006年

原著論文

• "Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo"
  Hidehito Kuroyanagi, Tetsuo Kobayashi, Shohei Mitani, Masatoshi Hagiwara
  Nature Methods 3, 11, 909-915 (2006).

  • DOI: 10.1038/nmeth944

2004年

書籍分担執筆

• "誘導的遺伝子発現とシグナル伝達"
  黒柳秀人／萩原正敏
  キーワードで理解する 転写イラストマップ（田村隆明／編）（羊土社）, 95-106 (2004).

  • URL