研究業績

原著論文

2025年

• "Q373fs variant of RBM20 affects splicing and expression of cardiac-related genes and cardiac function: Human sudden death case and mouse experiments. "
  Aya Miura, Takuma Yamamoto*, Mai Imasaka, Michihiko Sugimoto, Yoshiro Naito, Hiroshi Nishiura, Minori Nishiguchi, Kazuhisa Funayama, Yuri Yamasu, Akihide Koyama, Hisakazu Takatsuka, Hidehito Kuroyanagi, Masaki Ohmuraya, Hajime Nishio
  Human Molecular Genetics in press (2025).
• "UNC5B is an isoform-dependent target for ectodomain shedding."
  Kotaro Sugimoto, Eichi Watabe, Mio Takuma, Kaname Nagahara, Toshinori Sawano, Mihoko Kajita, Junichi Takagi, Hidehito Kuroyanagi, Kyoko Shirakabe.
  The Journal of Biochemistry 178, 4, 267–275 (2025).

  • DOI: 10.1093/jb/mvaf043
• "Cytoplasmic mutant RBM20 causes arrhythmogenicity in murine atria."
  Kensuke Ihara, Satoshi Iwamiya, Masaki Ikuta, Yurie Soejima, Yuichi Hiraoka, Atsushi Nakano, Susumu Minamisawa, Tetsushi Furukawa, Hidehito Kuroyanagi, Tetsuo Sasano
  Journal of Molecular and Cellular Cardiology 205, 1-12 (2025).

  • DOI: 10.1016/j.yjmcc.2025.06.001
• "Profiling of RBM20-Regulated CaMKIIδ Splice Variants Across the Heart, Skeletal Muscle, and Olfactory Bulbs."
  Yui MAEDA, Yuri YAMASU and Hidehito KUROYANAGI
  Genes to Cells 30, 3, e70021 (2025).

  • DOI: 10.1111/gtc.70021

2023年

• "Alternative splicing of a single exon causes a major impact on the affinity of Caenorhabditis elegans tropomyosin isoforms for actin filaments."
  Shoichiro Ono, Eichi Watabe, Keita Morisaki, Kanako Ono, Hidehito Kuroyanagi.
  Frontiers in Cell and Developmental Biology 11, 1208913 (2023).

  • DOI: 10.3389/fcell.2023.1208913
• "Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification."
  Hirotatsu Imai, Daisuke Utsumi, Hidetsugu Torihara, Kenzo Takahashi, Hidehito Kuroyanagi, Akio Yamashita.
  Nucleic Acids Research 51, 14, e76 (2023).

  • DOI: 10.1093/nar/gkad545
• "Structure of the Caenorhabditis elegans m6A methyltransferase METT10 that regulates SAM homeostasis."
  Jue Ju, Tomohiko Aoyama, Yuka Yashiro, Seisuke Yamashita, Hidehito Kuroyanagi, Kozo Tomita.
  Nucleic Acids Research 51, 5, 2434–2446 (2023).

  • DOI: 10.1093/nar/gkad081

2022年

• "I536T variant of RBM20 affects splicing of cardiac structural proteins that are causative for developing dilated cardiomyopathy."
  Takuma Yamamoto, Rie Sano, Aya Miura, Mai Imasaka, Yoshiro Naito, Minori Nishiguchi, Kensuke Ihara, Naruhito Otani, Yoshihiko Kominato, Masaki Ohmuraya, Hidehito Kuroyanagi and Hajime Nishio.
  Journal of Molecular Medicine 100, 12, 1741–1754 (2022).

  • DOI: 10.1007/s00109-022-02262-8

2021年

• "Periostin Exon-21 Antibody Neutralization of Triple-Negative Breast Cancer Cell-Derived Periostin Regulates Tumor-Associated Macrophage Polarization and Angiogenesis."
  Tatsuya Fujikawa, Fumihiro Sanada, Yoshiaki Taniyama, Kana Shibata, Naruto Katsuragi, Nobutaka Koibuchi, Kaori Akazawa, Yuko Kanemoto, Hidehito Kuroyanagi, Kenzo Shimazu, Hiromi Rakugi and Ryuichi Morishita.
  Cancers 13, 20, 5072 (2021).

  • DOI: 10.3390/cancers13205072
• "Loss of Caenorhabditis elegans homologue of human MOB4 compromises life span, health life span and thermotolerance."
  Momotaj Jahan, Hiroaki Iwasa, Hidehito Kuroyanagi, Yutaka Hata.
  Genes to Cells 26, 10, 798-806 (2021).

  • URL
  • DOI: 10.1111/gtc.12891
• "m6A-mediated alternative splicing coupled with nonsense-mediated mRNA decay regulates SAM synthetase homeostasis."
  Eichi Watabe, Marina Togo-Ohno, Yuma Ishigami, Shotaro Wani, Keiko Hirota, Mariko Kimura-Asami, Sharmin Hasan, Satomi Takei, Akiyoshi Fukamizu, Yutaka Suzuki, Tsutomu Suzuki, Hidehito Kuroyanagi.
  The EMBO Journal 40, e106434 (2021).

  • URL
  • DOI: 10.15252/embj.2020106434

2020年

• "Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs."
  Jay W Schneider, Saji Oommen, Muhammad Y Qureshi, Sean C Goetsch, David R Pease, Rhianna S Sundsbak, Wei Guo, Mingming Sun, Han Sun, Hidehito Kuroyanagi, Dennis A Webster, Alexander W Coutts, Kimberly A Holst, Brooks S Edwards, Nikolas Newville, Matthew A Hathcock, Tamene Melkamu, Francesca Briganti, Wu Wei, Maria G Romanelli, Scott C Fahrenkrug, Doug E Frantz, Timothy M Olson, Lars M Steinmetz, Daniel F Carlson, Timothy J Nelson, Wanek Program Preclinical Pipeline.
  Nature Medicine 26, 11, 1788–1800 (2020).

  • DOI: 10.1038/s41591-020-1087-x
• "A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice."
  Kensuke Ihara, Tetsuo Sasano, Yuichi Hiraoka, Marina Togo-Ohno, Yurie Soejima, Motoji Sawabe, Megumi Tsuchiya, Hidesato Ogawa, Tetsushi Furukawa, Hidehito Kuroyanagi.
  Scientific Reports 10, 17894 (2020).

  • DOI: 10.1038/s41598-020-74800-8

2018年

• "Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner"
  Eichi Watabe, Shoichiro Ono, Hidehito Kuroyanagi
  Cytoskeleton (Hoboken) 75, 10, 427-436 (2018).

  • DOI: 10.1002/cm.21489
• "UNC119 is a binding partner of tumor suppressor Ras-association domain family 6 and induces apoptosis and cell cycle arrest by MDM2 and p53"
  Hiroaki Iwasa, Aradhan Sarkar, Takanobu Shimizu, Takeru Sawada, Shakhawoat Hossain, Xiaoyin Xu, Junichi Maruyama, Kyoko Arimoto-Matsuzaki, Kanchanamala Withanage, Kentaro Nakagawa, Hidetake Kurihara, Hidehito Kuroyanagi, Yutaka Hata
  Cancer Science 109, 9, 2767-2780 (2018).

  • DOI: 10.1111/cas.13706
• "Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization."
  Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura & Hidehito Kuroyanagi.
  Scientific Reports 8, 8970 (2018).

  • DOI: 10.1038/s41598-018-26624-w
• "Tropomyosin isoforms differentially affect muscle contractility in the head and body regions of the nematode Caenorhabditis elegans"
  Dawn E Barnes, Eichi Watabe, Kanako Ono, Euiyoung Kwak, Hidehito Kuroyanagi, Shoichiro Ono
  Molecular Biology of the Cell 29, 9, 1075-1088 (2018).

  • DOI: 10.1091/mbc.E17-03-0152

2016年

• "Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor"
  Masahiro Tomioka, Yasuki Naito, Hidehito Kuroyanagi, Yuichi Iino
  Nature Communications 7, 11645 (2016).

  • DOI: 10.1038/ncomms11645
• "Evolutionarily conserved autoregulation of alternative pre-mRNA splicing by ribosomal protein L10a"
  Satomi Takei, Marina Togo-Ohno, Yutaka Suzuki, Hidehito Kuroyanagi
  Nucleic Acids Research 44, 12, 5585-5596 (2016).

  • DOI: 10.1093/nar/gkw152

2014年

• "RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing"
  Kanako Kuwasako, Mari Takahashi, Satoru Unzai, Kengo Tsuda, Seiko Yoshikawa, Fahu He, Naohiro Kobayashi, Peter Güntert, Mikako Shirouzu, Takuhiro Ito, Akiko Tanaka, Shigeyuki Yokoyama, Masatoshi Hagiwara, Hidehito Kuroyanagi, Yutaka Muto
  Nature Structural & Molecular Biology 21, 778–786 (2014).

  • DOI: 10.1038/nsmb.2870
• "Comprehensive analysis of mutually exclusive alternative splicing in C. elegans"
  Hidehito Kuroyanagi, Satomi Takei & Yutaka Suzuki
  Worm 3, 1, e28459 (2014).

  • DOI: 10.4161/worm.28459

2013年

• "CELF family RNA-binding protein UNC-75 regulates two sets of mutually exclusive exons of the unc-32 gene in neuron-specific manners in Caenorhabditis elegans."
  Hidehito Kuroyanagi, Yohei Watanabe, Masatoshi Hagiwara.
  PLoS Genetics 9, 2, e1003337 (2013).

  • DOI: 10.1371/journal.pgen.1003337
• "Position-dependent and neuron-specific splicing regulation by the CELF family RNA-binding protein UNC-75 in Caenorhabditis elegans"
  Hidehito Kuroyanagi, Yohei Watanabe, Yutaka Suzuki, Masatoshi Hagiwara
  Nucleic Acids Research 41, 7, 4015–4025 (2013).

  • DOI: 10.1093/nar/gkt097
• "Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans"
  Hiroaki Iwasa, Sainawaer Maimaiti, Hidehito Kuroyanagi, Shodai Kawano, Kazutoshi Inami, Shikshya Timalsina, Mitsunobu Ikeda, Kentaro Nakagawa, Yutaka Hata
  Experimental Cell Research 319, 7, 931-945 (2013).

  • DOI: 10.1016/j.yexcr.2013.01.020
• "Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1"
  Hiroaki Iwasa, Hidehito Kuroyanagi, Sainawaer Maimaiti, Mitsunobu Ikeda, Kentaro Nakagawa, Yutaka Hata
  Experimental Cell Research 319, 3, 1-11 (2013).

  • DOI: 10.1016/j.yexcr.2012.10.008

2012年

• "Muscle-specific splicing factors ASD-2 and SUP-12 cooperatively switch alternative pre-mRNA processing patterns of the ADF/cofilin gene in Caenorhabditis elegans"
  Genta Ohno, Kanako Ono, Marina Togo, Yohei Watanabe, Shoichiro Ono, Masatoshi Hagiwara, Hidehito Kuroyanagi
  PLoS Genetics 8, 10, e1002991 (2012).

  • DOI: 10.1371/journal.pgen.1002991

2010年

• "Dual role of the carboxyl-terminal region of pig liver L-kynurenine 3-monooxygenase: mitochondrial-targeting signal and enzymatic activity"
  Kumiko Hirai, Hidehito Kuroyanagi, Yoshitaka Tatebayashi, Yoshitaka Hayashi, Kanako Hirabayashi-Takahashi, Kuniaki Saito, Seiich Haga, Tomihiko Uemura, Susumu Izumi
  The Journal of Biochemistry 148, 6, 639-650 (2010).

  • DOI: 10.1093/jb/mvq099
• "Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans"
  Hidehito Kuroyanagi, Genta Ohno, Hiroaki Sakane, Hiroyuki Maruoka, Masatoshi Hagiwara
  Nature Protocols 5, 9, 1495–1517 (2010).

  • DOI: 10.1038/nprot.2010.107

2008年

• "STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo"
  Genta Ohno, Masatoshi Hagiwara, Hidehito Kuroyanagi
  Genes & Development 22, 3, 360-374 (2008).

  • DOI: 10.1101/gad.1620608

2007年

• "The Fox-1 family and SUP-12 coordinately regulate tissue-specific alternative splicing in vivo"
  Hidehito Kuroyanagi, Genta Ohno, Shohei Mitani, Masatoshi Hagiwara
  Molecular and Cellular Biology 27, 24, 8612-8621 (2007).

  • DOI: 10.1128/MCB.01508-07
• "The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans"
  Keiko Gengyo-Ando, Hidehito Kuroyanagi, Tetsuo Kobayashi, Motohide Murate, Kazushi Fujimoto, Shigeo Okabe, Shohei Mitani
  EMBO Reports 8, 2, 152-157 (2007).

  • DOI: 10.1038/sj.embor.7400882

2006年

• "Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo"
  Hidehito Kuroyanagi, Tetsuo Kobayashi, Shohei Mitani, Masatoshi Hagiwara
  Nature Methods 3, 11, 909-915 (2006).

  • DOI: 10.1038/nmeth944