Material

Here are some information about useful vectors we reported in Nature Methods (2006), Nature Protocols (2010) and others!

Constructing Expression Vectors with GATEWAY Technologies

The vectors described here are designed to utilize Gateway system (invitrogen).

Detailed description and protocols for constructing fluorescent protein expression vectors are available in our article in Nature Protocols (2010).

For general information about Gateway cloning and Multisite Gateway System, please visit the provider's website.

References

Kuroyanagi H, Kobayashi T, Mitani S, Hagiwara M.

Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo.

Nature Methods 3: 909-915, 2006.

Kuroyanagi H, Ohno G, Sakane H, Maruoka H, Hagiwara M.

Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans.

Nature Protocols 5: 1495-1517, 2010.

Satomi Takei, Marina Togo-Ohno, Yutaka Suzuki, Hidehito Kuroyanagi.

Evolutionarily conserved autoregulation of alternative pre-mRNA splicing by ribosomal protein L10a.

Nucleic Acids Research 44: 5585-5596, 2016.

Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura & Hidehito Kuroyanagi.

Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization.

Scientific Reports 8: 8970, 2018.

Tatsuya Fujikawa, Fumihiro Sanada, Yoshiaki Taniyama, Kana Shibata, Naruto Katsuragi, Nobutaka Koibuchi, Kaori Akazawa, Yuko Kanemoto, Hidehito Kuroyanagi, Kenzo Shimazu, Hiromi Rakugi and Ryuichi Morishita.

Periostin Exon-21 Antibody Neutralization of Triple-Negative Breast Cancer Cell-Derived Periostin Regulates Tumor-Associated Macrophage Polarization and Angiogenesis.

Cancers 13: 5072, 2021.

Acknowledgement

The vectors listed here are constructed by modifying materials derived from researchers and/or providers listed here.

Andrew Fire (Stanford Univ) 

Atsushi Miyawaki (RIKEN) 

Masaaki Muramatsu (TMDU) 

Roger Y. Tsien (UC, San Diego) 

Caenorhabditis Genetics Center (CGC)

Clontech 

Invitrogen 

TaKaRa