Material
Entry Vectors
pENTR Vectors (Entry Vectors) provide a fluorescent protein cassette for constructing a variety of Gateway Expression vectors.
pENTR-L1/L2 Vectors
pENTR-L1/L2 vectors provide a fluorescent protein cDNA cassette for expression of an unfused fluorescent protein.
| Vector Name | Color | Features |
| pENTR-L1-ECFP-L2 | Cyan | Initiation/Termination codons |
| pENTR-L1-EGFP-L2 | Green | Initiation/Termination codons |
| pENTR-L1-mRFP-L2 | Red | Initiation/Termination codons |
| pENTR-L1-Venus-L2 | Yellow | Initiation/Termination codons |
| pENTR-L1-mCherry-L2 | Red | Initiation/Termination codons |
pENTR-L1/R5 Vectors
pENTR-L1/R5 vectors provide a fluorescent protein cDNA cassette for expression of a fusion protein with an N-terminal fluorescent protein.
| Vector Name | Color | Features |
| pENTR-L1-EGFP-R5 | Green | With initiation codon No termination codon |
| pENTR-L1-mRFP-R5 | Red | With initiation codon No termination codon |
| pENTR-L1-kEGFP(RPL3I8@426)s-R5 | Green | Initiation/Termination codons Artificial intron from human RPL3 |
| pENTR-L1-kEGFP(RPL3I8@606)s-R5 | Green | Initiation/Termination codons Artificial intron from human RPL3 |
| pENTR-L1-kEGFP(RPL3I8@606)-R5 | Green | With initiation codon No termination codon Artificial intron from human RPL3 |
pENTR-L5/L2 Vectors
pENTR-L5/L2 vectors provide a fluorescent protein cDNA cassette for expression of a fusion protein with a C-terminal fluorescent protein.
| Vector Name | Color | Features |
| pENTR-L5-ECFP-L2 | Cyan | Initiation/Termination codons |
| pENTR-L5-EGFP-L2 | Green | Initiation/Termination codons |
| pENTR-L5-Venus-L2 | Yellow | Initiation/Termination codons |
| pENTR-L5-HcRed-L2 | Red | Initiation/Termination codons |
| pENTR-L5-mRFP-L2 | Red | Initiation/Termination codons |
| pENTR-L5-GGS6-EGFP-L2 | Green | No initiation codon 6x(Gly-Gly-Ser) linker |
| pENTR-L5-(+1)GGS6-EGFP-L2 | Green | No initiation codon 6x(Gly-Gly-Ser) linker Frameshift (1-base insertion) |
| pENTR-L5-(+2)GGS6-EGFP-L2 | Green | No initiation codon 6x(Gly-Gly-Ser) linker Frameshift (2-base insertion) |
| pENTR-L5-GGS6-Venus-L2 | Yellow | No initiation codon 6x(Gly-Gly-Ser) linker |
| pENTR-L5-GGS6-mCherry-L2 | Red | No initiation codon 6x(Gly-Gly-Ser) linker |
| pENTR-L5-(+1)GGS6-mCherry-L2 | Red | No initiation codon 6x(Gly-Gly-Ser) linker Frameshift (1-base insertion) |
| pENTR-L5-(+2)GGS6-mCherry-L2 | Red | No initiation codon 6x(Gly-Gly-Ser) linker Frameshift (2-base insertion) |
| pENTR-L5-(+1)GGS6-ECFP-L2 | Cyan | No initiation codon 6x(Gly-Gly-Ser) linker Frameshift (1-base insertion) |
| pENTR-L5-GGS6-EGFP-(+2)2A-mCherry-L2 | Green/Red | No initiation codon GFP with a 6x(Gly-Gly-Ser) linker or RFP with a 2A linker |
| pENTR-L5-P2A-EGFP-L2 | Green | No initiation codon P2A linker |
| pENTR-L5-(+1)P2A-EGFP-L2 | Green | No initiation codon P2A linker Frameshift (1-base insertion) |
| pENTR-L5-(+2)P2A-EGFP-L2 | Green | No initiation codon P2A linker Frameshift (2-base insertion) |
| pENTR-L5-P2A-mCherry-L2 | Red | No initiation codon P2A linker |
| pENTR-L5-(+1)P2A-mCherry-L2 | Red | No initiation codon P2A linker Frameshift (1-base insertion) |
| pENTR-L5-(+2)P2A-mCherry-L2 | Red | No initiation codon P2A linker Frameshift (2-base insertion) |
