pDEST Vectors provide a promoter and a 3' cassette for constructing a variety of Gateway Expression vectors.

The Destination Vectors listed here include a promoter and a 5'UTR located upstream from attR1, and a 3' cassette for 3'UTR and transcription termination located downstream from attR2. Some Vectors include an N-terminal FLAG or HA tag for expression of tagged proteins. 

For Worms

Here are pDEST Vectors for constructing Expression Vectors for nematodes. Most of the Vectors listed here were constructed by inserting a promoter fragment into the Multiple Cloning Site (MCS) of pDEST-PL (Nat Protoc, 2010; schematically described below), which is converted from pPD49.26 from Prof. Andy Fire.

For Cultured Cells

Here are pDEST Vectors for constructing Expression Vectors for cultured cells.

For Bacteria

Here are pDEST Vectors for constructing cold-shock-inducible Expression Vectors for E. coli.